Module I: Leishmania parasite, diagnosis, molecular identification and drugs screening |
Student
Presentations (will be posted after presentations) |
Monday July 24th - Friday July 28th:
Ibrahim Abbasi and Charles Jaffe:Lab Manual:
Leishmania parasite, diagnosis, molecular identification and drugs screening
Sequencing ResultsDiscussion points
Useful Links:
EBI Bioinformatic Tools (ie. sequence alignment and phylogenetic tree building)
iTOL (Phylogenetic Tree building)
IC50 calculation (description and excel file)
Free Software:
MEGA (Molecular Evolutionary Genetics Analysis)
Reading material:
Microsatellite marker identification using genome screening and restriction-ligation
Identification of Blood Meals Imbibed by Phlebotomine Sand Flies Using Cytochrome b PCR and Reverse Line BlottingRecent updates and perspectives on leishmaniasis
Leishmania donovani: Intraspecific Polymorphisms of Sudanese Isolates Revealed by PCR-based Analyses and DNA Sequencing
Comparison of PCR Assays for Diagnosis of Cutaneous Leishmaniasis
Leishmaniasis in the Middle East: Incidence and Epidemiology
Multilocus microsatellite typing of Leishmania and clinical applications: a review
Evaluation of PCR procedures for detecting and quantifying Leishmania donovani DNA in large numbers of dried human blood samples from a visceral leishmaniasis focus in Northern Ethiopia
Cutaneous Leishmaniasis: Recent Developments in Diagnosis and Management
Leishmaniases in Maghreb: An endemic neglected disease
Developments in Diagnosis of Visceral Leishmaniasis in the Elimination Era